Single-cell RNA sequencing characterization of Holstein cattle blood and milk immune cells during a chronic Staphylococcus aureus mastitis infection

All cows developed chronic mastitis infections, confirmed by bacterial plating and somatic cell counts (SCC), following S. aureus challenge with strain Newbould 305. Post-infection, animals averaged 2.82 × 10⁶ ± 1.08 × 10⁶ somatic…