Molecular basis for the calcium-dependent activation of the ribonuclease EndoU

Cell culture

VL3-3M2 mouse thymic lymphoma cells³⁴ (obtained from Stephen Smale’s lab, UCLA) were cultured in RPMI 1640 (Corning) supplemented with 10 mM HEPES, 50 μM β-mercaptoethanol, 1× penicillin/streptomycin, and 10% fetal bovine…