Cell culture
We purchased SUM149 cells from the ATCC (Manassas, VA) and cultured cells in F-12 media supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (Pen/Strep) (Thermo Fisher Scientific, Waltham, MA), 1% GlutaMAX…
Substrate stiffness regulates triple-negative breast cancer signaling through CXCR4 receptor dynamics
