Complementary cytoskeletal feedback loops control signal transduction excitability and cell polarity

Cells and plasmids

Dictyostelium discoideum cells were cultured in HL5 media⁹¹ for a maximum of 2 months after thawing from frozen stock. AX3 cells were obtained from the R. Kay laboratory (MRC Laboratory of Molecular Biology, UK), abnABC⁻ cells…