Computationally unmasking each fatty acyl C=C position in complex lipids by routine LC-MS/MS lipidomics

Cell culture

The murine RAW264.7 cell line was obtained from the American Type Culture Collection (ATCC #TIB-71). RAW264.7 cells were cultured as described elsewhere¹⁴ in complete DMEM (containing 1% v/v P/S, 10% v/v FBS, and 1% v/v glutamine) in…