In-cell NMR reveals the first direct observation of endogenous interaction between HIV Tat protein and Tat RNA aptamer in human cells

Cell culture and expression of HIV Tat protein in HeLa cells

HeLa cells (ATCC^® CCL-2™), a human cervical epithelial carcinoma cell line, were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) containing 10% heat-inactivated fetal…