Protein purification
αSyn was expressed in DE3 Star E. coli cell lines (Thermo Fisher Scientific). Cells were grown in LB media at 37 °C, 180 rpm until reaching an optical density at 600 nm of 0.6–0.8. Protein expression was induced by…
Stepwise recruitment of chaperone Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibril disassembly
