Mammalian expression constructs
GCase wt was expressed using a commercially available plasmid (pCMV3-GBA-wt, Sino Biological Inc., Peking, China, #HG12038-UT). The expression construct for the secreted LIMP-2 ectodomain (sLIMP-2) was described…
Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
