Membrane-domain compartmentalization of active GPCRs by β-arrestins through PtdIns(4,5)P2 binding

Reagents and plasmids

AVP and isoproterenol (ISO) were purchased from Sigma-Aldrich. Tetramethylrhodamine (TMR)-labeled HiBiT peptide (TMR–HiBiT: TMR-VSGWRLFKKIS) was synthesized in Genscript. SaraFluor650-labeled Flag–HiBiT²³ (SF650–FiBiT:…