Cloning and bacterial strain handling
All plasmids and phage (Supplementary Table 1) were cloned by Gibson Assembly of PCR fragments generated using Q5 DNA polymerase (NEB). All primers (Supplementary Table 2) were ordered from IDT. Three E. coli…
All plasmids and phage (Supplementary Table 1) were cloned by Gibson Assembly of PCR fragments generated using Q5 DNA polymerase (NEB). All primers (Supplementary Table 2) were ordered from IDT. Three E. coli…