Two successive oligomeric Munc13 assemblies scaffold vesicle docking and SNARE assembly to support neurotransmitter release

Protein constructs

The following cDNA constructs previously described^20,26 were used in this study: full-length VAMP2 (mouse His⁶-SUMO-VAMP2, residues 1-116); full-length SNAP25 (mouse His⁶-SNAP25b, residue 1-206); Synaptotagmin (rat Synaptotagmin…